| 主权项 |
1. A method of diagnosing a subject as having or at risk of having pre-diabetes or diabetes, comprising:
a) obtaining a blood sample from the subject; b) isolating erythrocytes having erythrocyte peptides from the blood sample, the erythrocyte peptides comprising at least one of the amino acid sequences selected from SEQ ID NOs: 1, 2, 3, 5, 7, 8, 15-17, 24, 26, 28-32 or 37, and optionally isolating the erythrocyte peptides having the selected amino acid sequences from the isolated erythrocytes; c) performing an immunoassay to detect a level of O-linked N-acetyl-glucosamine (O-GlcNAc) modification of an amino acid residue within the amino acid sequence of at least one of the selected amino acid sequences of the erythrocyte peptides, the immunoassay comprising contacting the isolated erythrocytes or erythrocyte peptides with at least one first antibody that specifically binds to O-G1cNAc present on the amino acid residue of the at least one selected amino acid sequence of the erythrocyte peptides, wherein the at least one first antibody does not specifically bind other O-G1cNAc-modified peptides not having the at least one selected amino acid sequence, and with at least one second antibody that specifically binds the amino acid residue of the at least one selected amino acid sequence in a non-O-linked glycosylated state and detecting the first and second antibody bound to the O-G1cNAc-modified and non-O-glycosylated amino acid residue, respectively, of the at least one selected amino acid sequence; and d) calculating a ratio of bound first antibody to bound second antibody and comparing the ratio to that of a corresponding normal sample, wherein if the ratio is different from the ratio of the corresponding normal sample a diagnosis of pre-diabetes or diabetes, or an increased risk therefore, is indicated for the subject. |
