Apparatus and methods for integrated sample preparation, reaction and detection

摘要

An apparatus includes a housing, a reaction vial and a transfer mechanism. The housing defines a first flow path and a second flow path. The housing has transfer port defining an opening in fluid communication with the second flow path and a volume outside of the housing. The transfer port includes a flow control member to limit flow through the opening. The reaction vial is coupled to the housing and defines a reaction volume, which is in fluid communication with the transfer port via the second flow path. The transfer mechanism is configured to transfer a sample from an isolation chamber of an isolation module to the reaction chamber via at least the first flow path when the transfer mechanism is actuated. The transfer mechanism configured to produce a vacuum in the reaction vial to produce a flow of a sample from the isolation chamber to the reaction volume.

主权项

1. An apparatus, comprising:
a housing defining a first flow path and a second flow path, the housing having a flow cell portion defining a detection volume, wherein the flow cell portion is located within an extension of the housing; a reaction vial coupled to the housing, the reaction vial defining a reaction volume, the reaction volume in fluid communication with the detection volume via the first flow path; and a transfer mechanism in fluid communication with the detection volume via the second flow path, the transfer mechanism configured to transfer a sample from the reaction volume to the detection volume when the transfer mechanism is actuated; an optics module interfaced with the extension, wherein:
the apparatus is configured to allow the sample to flow within the first flow path in a first direction into the detection volume while a second portion of the sample flows within the second flow path in a second direction opposite the first direction;the optics module comprises a magnet.