发明名称 |
FREMGANGSMÅDE TIL REDUKTION AF HETEROGENITET AF ANTISTOFFER OG FREMGANGSMÅDE TIL FREMSTILLING AF DISSE ANTISTOFFER |
摘要 |
The present disclosure relates to a method of reducing heterogeneity in antibodies during culturing, wherein the heterogeneity is due to proportion of charge variant of the antibody. The disclosure also comprises a process of growing cells in a cell culture system that results in antibodies with the reduced heterogeneity. In one embodiment antibody heterogeneity is reduced by the addition of divalent transitional metal ions such as zinc (Zn2+) to the cell culture medium. In another embodiment antibody heterogeneity is reduced by decreasing the osmolality of the cell culture medium. |
申请公布号 |
DK2702164(T3) |
申请公布日期 |
2016.02.01 |
申请号 |
DK20120777325T |
申请日期 |
2012.04.27 |
申请人 |
BIOCON RESEARCH LIMITED |
发明人 |
SRIVASTAVA, RUCHIKA;HEMDEV, SNEHA LAKSHMANDAS;BHATNAGAR, ANKUR;DESAN, SARAVANAN;GOEL, ANUJ;IYER, HARISH |
分类号 |
C12P21/08;A61K39/395;C07K16/00 |
主分类号 |
C12P21/08 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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