TRANSGENIC PLANTS EXPRESSING A NOVEL POLYPEPTIDE WHICH IS ENCODED BY A SYNTHETIC GENE THAT CONTAINS IMMUNOPROTECTIVE SEQUENCES FROM CORINEBACTERIUM DIPHTERIAE, BORDETELLA PERTUSSIS AND CLOSTRIDIUM TETANI EXOTOXINS.

摘要

Provided is a plant produced vaccine acting against diphtheria, pertussis and tetanus, which is best known as DPT vaccine. The vaccine includes an immunogenic complex resulting from the transformation of tomatoe with a chimeric gene optimised to be expressed in plants, which encodes a novel recombinant polypeptide that is not available in nature and which contains the main immunoprotective epitopes of diphtheria, pertussis and tetanus. In addition, methods for building the genetic transformation vector are described, as well as regeneration methods based on the culture of vegetable tissues. The results of the antibody production in tests performed to mice are also shown, which may be useful for the mammals immunisation.