摘要 |
The invention relates to a method for detection of at least two cytokeratins selected from the group consisting of cytokeratin 8, 18 and 19, and/or soluble fragments thereof, in a sample, comprising the steps of contacting said sample with a solid phase having immobilized thereon a first antibody having specificity for cytokeratin 8, a second antibody having specificity for cytokeratin 18 and, optionally,a third antibody having specificity for a first epitope of cytokeratin 19; allowing cytokeratin and/or soluble fragments thereof in said sample to bind to said first, second and optionally third antibodies thereby forming complexes; contacting said complexes with a first labelled antibody having specificity for a dimer of cytokeratin 8 and 18 and optionally a second labelled antibody having specificity for a second epitope of cytokeratin 19; allowing said first and second labelled antibodies to bind to said complexes; and detecting said first labelled antibody and said second labelled antibody bound to said complexes. The invention further relates to a method for quantitative determination of soluble fragments of at least two of cytokeratin 8, 18 and 19 in a sample and to a kit-of-parts for performing the method according to the invention. |
主权项 |
1. A method for detection of at least two cytokeratins selected from the group consisting of cytokeratin 8, 18 and 19, and/or soluble fragments thereof, in a sample, comprising the steps of
contacting said sample with a solid phase having immobilized thereon a first antibody having specificity for cytokeratin 8, a second antibody having specificity for cytokeratin 18 and optionally a third antibody having specificity for a first epitope of cytokeratin 19; allowing cytokeratin and/or soluble fragments thereof in said sample to bind to said first, second and optionally third antibodies thereby forming complexes; contacting said complexes with a first labelled antibody having specificity for a dimer of cytokeratin 8 and 18 and optionally a second labelled antibody having specificity for a second epitope of cytokeratin 19; allowing said labelled antibodies to bind to said complexes; and detecting said labelled antibodies bound to said complexes. |