| 摘要 |
The present invention relates to a recombinant microorganism comprising nucleotide sequence(s) encoding: a polypeptide having ent-copalyl pyrophosphate synthase activity; a polypeptide having ent-Kaurene synthase activity; a polypeptide having ent-Kaurene oxidase activity; and a polypeptide having kaurenoic acid 13-hydroxylase activity, a polypeptide capable of catalyzing the addition of a glucose at the C-13 position of steviol, a polypeptide capable of catalyzing the addition of a glucose at the C-13 position of steviolmonoside or at the C-19 position of rebaudioside A, a polypeptide capable of catalyzing the addition of a glucose at the C-19 position of steviolbioside; and a polypeptide capable of catalyzing addition of a glucose at the C-13 position of stevioside or at the C-19 position of rebaudioside D, whereby expression of the nucleotide sequence(s) confer(s) on the microorganism the ability to produce at least rebaudioside M. Such a recombinant microorganism may be used in a method of production of rebaudioside M. |
