发明名称 |
METHOD OF MEASURING HUMAN CYP3A INDUCIBILITY |
摘要 |
A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells.;The present invention ensures convenient and accurate evaluation of human CYP3A inducibility upon administration of a test drug to a human subject, providing accurate evaluation in terms of the efficacy of the test drug, occurrence of side effects, disappearance of the drug effect, etc. |
申请公布号 |
US2016025706(A1) |
申请公布日期 |
2016.01.28 |
申请号 |
US201514875725 |
申请日期 |
2015.10.06 |
申请人 |
SEKISUI MEDICAL CO., LTD. |
发明人 |
YAMAZOE Yasushi;NAGATA Kiyoshi |
分类号 |
G01N33/50 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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主权项 |
1. A method for measuring human CYP3A inducibility upon administration of a test drug, characterized in that a non-human animal to which a test drug is administered or a population of human cells cultured in a medium containing a test drug is infected with viruses (A) and (B); virus (A) being an adenovirus which is used as a vector and engineered by incorporating thereto a detectable reporter gene and at least 3 human PXR binding regions falling within an untranslated region of a human CYP3A gene, and virus (B) being an adenovirus which is used as a vector and engineered by incorporating thereto a human PXR cDNA; and subsequently expression level of the reporter gene is determined in the non-human animal or the cultured human cells. |
地址 |
Tokyo JP |