METHODS AND COMPOSITIONS FOR IN VIVO IMMUNE STIMULATION AND ANTIGEN PRODUCTION

摘要

Disclosed are compositions and methods comprising a vector and a reverse genetics competent unit. The vector may comprise baculovirus expression vectors, bacmids, vaccinia virus and synthetic vectors and the reverse genetics competent unit may comprise pathogenic units necessary for producing pathogens de novo from a nucleotide-based vector. In certain embodiments, the reverse genetics competent unit comprises pathogenic units necessary for producing pathogens de novo from a nucleotide-based vector for influenza virus.

主权项

1. A method of in vivo synthesis of a vaccine, comprising, providing to at least one cell of a subject a vector comprising an exogenous DNA construct encoding at least a portion of a pathogenic agent or an oncogenic protein, wherein one or more antigenic peptides encoded by the DNA construct are expressed in the cell and stimulate an immune response in the subject to the pathogenic agent.