METHOD FOR THE BIOTECHNOLOGICAL PRODUCTION OF FLAVONE GLYCOSIDE DIHYDROCHALCONES

摘要

The invention relates to a method for producing flavone glycoside dihydrochalcones, having the following steps: (a) providing a transgenic microorganism containing (i) a first nucleic acid portion (A) containing a gene which codes for a bacterial chalcone isomerase and (ii) a second nucleic acid portion (B) containing a gene which codes for a bacterial enoate reductase, (b) adding one or more flavone glycosides to the transgenic microorganism, (c) cultivating the transgenic microorganism under conditions which allow the simultaneous isomerization and reduction of the flavone glycoside into the flavone glycoside dihydrochalcone, and optionally (d) isolating and purifying the final product, wherein the nucleic acid portion (A) (1) is a nucleotide sequence according to SEQ ID NO:1, in which the nucleic acid portion (A') according to SEQ ID NO:3 has been cut out, or (2) is an amino acid sequence according to SEQ ID NO:2, in which the amino acid portion (A') according to SEQ ID NO:4 has been cut out.