METHODS AND USES FOR MOLECULAR TAGS

摘要

Methods and uses for molecular tags are disclosed. Molecular tags may be attached to nucleic acid molecules. The attachment of the nucleic acid molecules prior to PCR amplification and sequencing improves the accuracy of genetic analysis and detection of genetic variations and diversity. Molecular tags may also be used for detection of drug-resistant variants. Methods for using molecular tags for determining and correcting PCR errors and/or sequencing error are also disclosed.

主权项

1. A method for analyzing a plurality of nucleic acid molecules comprising:
(a) attaching a plurality of primers comprising a Primer ID to a plurality of nucleic acid molecules in a sample to generate tagged-nucleic acid templates, wherein (i) the plurality of nucleic acid molecules comprises 10 or more nucleic acid templates, and (ii) each tagged nucleic acid template is attached to a unique Primer ID; (b) amplifying the tagged nucleic acid templates to produce tagged amplicons; and (c) detecting the tagged amplicons, thereby analyzing the plurality of nucleic acid molecules, wherein analyzing the plurality of nucleic acid molecules comprises determining, with the aid of a computer processor, (i) PCR resampling in an amplification reaction; or (ii) a PCR error rate and/or sequencing error rate.