METHOD AND APPARATUS FOR SEPARATING QUALITY LEVELS IN SEQUENCE DATA AND SEQUENCING LONGER READS

摘要

Sequencing reads from a measurement system may be classified based on quality scores associated with the measurement system, and corresponding error characteristics may be provided. The sequencing reads may correspond to at least one of deoxyribonucleic acid (DNA), complementary DNA (cDNA), or ribonucleic acid (RNA).

主权项

1. A method of processing sequencing reads, the method comprising:
accessing a plurality of sequencing reads associated with a measurement system, each sequencing read including a sequence of base values, and one or more locations of each sequencing read being associated with a quality score that characterizes operations of the measurement system at the one or more locations; specifying one or more quality conditions based on values of the quality score; using the one or more quality conditions to specify one or more quality classifications for the sequencing reads, each quality classification being based on satisfying at least one corresponding quality condition at locations of the sequencing reads; and providing an error characteristic corresponding to each quality classification.