BIOACTIVE MOLECULES PRODUCED BY PROBIOTIC BACTERIA AND METHODS FOR ISOLATING AND USING THE SAME

摘要

The present invention is a method for isolating bioactive molecules secreted by probiotic bacteria such as Lactobacillus rhamnosus, and methods for using such bioactive molecules to activate NOD2, decrease expression of inflammatory molecules, inhibit replication of human immunodeficiency virus (HIV), stimulate expression of Apolipoprotein A-IV, modulate diet-associated weight gain and prevent mucosal transmission of HIV.

主权项

1. A method for isolating bioactive small molecules from probiotic bacteria comprising
(a) culturing pure probiotic bacteria in nutrient-rich medium for approximately 18 to 24 hours; (b) separating the probiotic bacteria from the nutrient-rich medium; (c) washing the probiotic bacteria with water to remove residual nutrient-rich medium; (d) culturing the probiotic bacteria in a second medium consisting of water or water and a monosaccharide for approximately seven to 24 hours; (e) separating the probiotic bacteria from the second medium to yield a bacteria-free supernatant containing bioactive small molecules; (f) applying the bacteria-free supernatant to an activated porous graphitized carbon (PGC) matrix; (g) washing the PGC matrix to remove unbound material; and (h) eluting bound bioactive small molecules by sequential application of
(i) a polar solvent and(ii) a polar solvent with an acidic modifier thereby isolating bioactive small molecules secreted into a bacterial culture medium.