SAMPLE CONCENTRATOR AND METHOD OF USE

摘要

The present disclosure provides an assembly for preparing a sample for analysis. The assembly comprises a hollow body comprising first opening, an outlet with a second opening, and a fluid pathway extending therebetween; a filter element operatively interposed in the fluid pathway; and a closure comprising a third opening and a chamber, the closure being slideably engaged with the outlet. The chamber and the outlet are dimensioned so that the outlet is sealingly engaged with the chamber. A method of using the assembly to detect a microorganism is also provided.

主权项

1. A method, comprising:
depositing a liquid sample suspected of containing a microorganism into a sample preparation device, the sample preparation device comprising:
a hollow body comprising first opening, an outlet with a second opening, and a fluid pathway extending therebetween; anda filter element operatively interposed in the fluid pathway; passing the liquid sample through the filter element to retain the microorganism; urging the outlet into a chamber configured to sealingly receive the outlet, the chamber containing a back-flush liquid;
wherein urging the outlet into the chamber comprises urging the outlet into the chamber to an extent sufficient to cause the back-flush liquid to contact the filter element; and analyzing a portion of the back-flush liquid to detect a presence or an absence of the microorganism.