METHODS FOR EVALUATING INSECT RESISTANCE IN A PLANT

摘要

Methods are provided for evaluating the insect resistance of a plant of interest. Specifically, methods are provided for high-throughput screening of soybean plants for resistance to kudzu bugs and stink bugs, including the brown marmorated stink bug and the southern green stink bug. In some embodiments, infesting emergent soybean seedlings with second instar stink bug nymphs and maintaining the nymphs in a closed environment with the seedling for only about 7 days allows evaluation of the stink bug resistance of a soybean variety of interest. Also provided are insect resistant plants, particularly plants resistant to kudzu bugs and stink bugs, as well as seeds produced by the resistant plants. Plants disclosed herein can be used to transfer the resistant trait into plant lines of interest.

主权项

1. A method of evaluating insect resistance in a plant comprising:
a) planting a seed and allowing said seed to germinate at least until plant emergence; b) infesting said plant with at least one insect nymph; c) maintaining said nymph in an enclosed environment for at least as many days as the nymph can survive without food; and d) evaluating insect resistance of said plant.