IDENTIFICATION OF ANTIBODIES SPECIFIC FOR LYSSAVIRUSES AND METHODS OF THEIR USE

摘要

Described herein is a method of identifying a monoclonal antibody (or antigen-binding fragment thereof) that specifically binds a plurality of lyssaviruses for use in post-exposure rabies prophylaxis or in the treatment of clinical rabies. The method includes using a naïve antibody phage display library to screen for phage clones that bind whole recombinant rabies virus or cells expressing glycoprotein from multiple lyssaviruses (such as RABV, MOKV and WCBV) and/or specifically bind recombinant glycoprotein from different lyssaviruses.

主权项

1. An isolated monoclonal antibody, or antigen-binding fragment thereof, that specifically binds at least two different lyssaviruses or that specifically binds glycoprotein from at least two different lyssaviruses, wherein the antibody or antigen-binding fragment thereof comprises a variable heavy (VH) domain complementarity determining region 1 (CDR1), CDR2 and CDR3, and wherein the CDR1, CDR2 and CDR3 are encoded by the CDR1, CDR2 and CDR3 nucleotide sequences of any one of SEQ ID NOs: 44-110.