| 发明名称 |
METHODS FOR PURIFYING ANTIBODIES |
| 摘要 |
A method for purifying a protein comprising an antibody, antibody fragment, or immunoglobulin single variable domain, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate; and c) eluting the protein from the superantigen immobilized on the solid support. |
| 申请公布号 |
US2016024146(A1) |
申请公布日期 |
2016.01.28 |
| 申请号 |
US201414775868 |
申请日期 |
2014.03.13 |
| 申请人 |
GLAXOSMITHKLINE INTELLECTUAL PROPERTY (NO.2) LIMITED |
发明人 |
GOKLEN Kent E.;SUDA Eric J.;UBIERA Antoni Raul |
| 分类号 |
C07K1/22 |
主分类号 |
C07K1/22 |
| 代理机构 |
|
代理人 |
|
| 主权项 |
1. A method for purifying a protein, from a solution containing at least one contaminant by superantigen chromatography comprising: a) adsorbing the protein to the superantigen immobilized on a solid support; b) removing the at least one contaminant by contacting the immobilized superantigen containing the adsorbed protein with a first wash buffer comprising an aliphatic carboxylate, wherein said aliphatic carboxylate comprises a 6-12 carbon backbone; and c) eluting the protein from the superantigen immobilized on the solid support. |
| 地址 |
Brentford Middlesex GB |
