MENSTRUAL STEMS CELLS FOR THE EFFICIENT SUPPORT AND EXPANSION OF CD34+ CD133+ HEMATOPOIETIC STEM CELLS IN VITRO

摘要

The invention relates to highly proliferative stem cell obtained from menstrual fluid. The menstrual stern cells (MenSCs) may be cultured in vitro and exhibit mesenchymal stem cell (MSC)-like properties and some culture and expansion advantages. This population of MenSCs, compared to the broadly studied bone marrow derived stem cells (BM-MSCs) out-performs bone marrow derived mesenchymal stem cells in proliferation rate and support of hematopoietic stem cell (HSC) expansion in vitro. MenSCs demonstrate to maintain their stem cell properties over 2 years, being genetically stable, without expressing surface differentiation markers, and they also show the ability to differentiate into adipocytes, chondrocytes and osteoblast cells. MenSCs can be easily and periodically obtained, isolated and cultured.

主权项

1. A method for culturing and proliferating hematopoietic stem cells, comprising the steps of:
(a) culturing hematopoietic stem cells; (b) culturing menstrual stem cells obtained from a human donor; (c) co-culturing the hematopoietic stem cells cultured in step (a) and the menstrual stem cells cultured in step (b), whereby the hematopoietic stem cells proliferate.