| 摘要 |
The invention relates to medicine. A method of correcting pathological human skin conditions related to aging includes a preliminary evaluation of a person's skin, selecting material, cultivating cells, separating a fibroblast culture, and separating cell material, wherein a first portion of the fibroblast culture is sent to be studied, and a second portion of the fibroblast culture is stored. The fibroblast culture is then analyzed by determining a DNA sequence and gene activity, the obtained results are compared to data from a standard group, and abnormalities in the fibroblast genome are identified. On the basis of the obtained genetic research data, a conclusion is made regarding a link between the identified abnormalities in the structure and/or function of a gene and changes in the patient's skin. Genetic constructs are created containing cDNA of those genes of the patient which exhibit altered activity or the DNA structure of which shows abnormalities in the patient, said constructs providing said genes with the structure and function of normal genes; the genetic constructs are introduced into a culture of the patient's fibroblasts, and modified autologous fibroblasts are then administered to the patient. |
