| 摘要 |
FIELD: biotechnologies.SUBSTANCE: recombinant DNA is offered, which provides in the composition of a plasmid vector the synthesis in cells of E.coli of a chimeric protein - apolipoprotein A-I of a human being, with the size of 852 bps. The recombinant DNA in series includes from 5'- end DNA fragment coding 6 a.o. of histidine with the size of 18 bps (SEQ ID NO 1), DNA fragment coding 6 a.o. pro-form of the protein apoA-I of a human being with the size of 18 bps (SEQ ID NO 2), DNA fragment coding N-end fragment from 15 a.o. of bull pancreatic RNAase A (SEQ ID NO 3) with the size of 45 bps, DNA fragment coding 6 a.o. pro-form- of the protein apoA-I of a human being with the size of 18 bps (SEQ ID NO 2), DNA fragment coding the site of the recognition of protease of a tobacco mosaic virus with the size of 21 bps (SEQ ID NO 4), mutant gene of a mature protein apoA-I of a human being with the size of 729 bps (SEQ ID NO 5). The yield of the chimeric protein of apolipoprotein A-I of a human being from 1 l of the culture of the cells E.coli is 182±32 mg that amounts 39.7±6.2 mg from 1 g of the biomass.EFFECT: veracity improvement.5 dwg, 3 tbl, 9 ex |
