摘要 |
Step S1: acquiring fluorescent images of frames in time-series. Each fluorescent image comprises pixels in which pixel data are acquired in the time-series. Step S2: setting analysis areas to the fluorescent images. Step S3: selecting the fluorescent images of two or more frames to be used in analysis. Step S4: extracting data pairs each comprising two pixels in which acquisition time intervals are the same in the analysis area of each of the fluorescent images selected in step S3, and performing product sum calculation of each of the data pairs for all of the selected images to calculate a correlation value. |