| 摘要 |
FIELD: bioengineering.SUBSTANCE: method of sensitivity testing of Mycobacterium tuberculosis to the pharmaceuticals, indicator use for the said method is suggested, where the indicator is tellurite or mixture of tellurite and urea, solid medium for the sensitivity test method, use of the solid medium, heating thermostat for the sensitivity test method and cultivation box for the sensitivity test method are also suggested. The method includes production of the rich samples with Mycobacterium tuberculosis, addition of the liquid culture medium to samples, heating and melting of the solid medium, mixing of liquid medium samples with melted solid medium, solidification of the liquid samples to obtain the solid samples for testing of the sensitivity to the pharmaceuticals using the paper strips, cultivation of the solid samples, indicator addition to the solid sample, and monitoring of the inhibition zone of the pharmaceutical as per level of indicator indication. The solid sample includes 50-100 ml of vitelline liquid, 2-8 g of soluble starch, 0.1-0.8 g of L-casein, 2-8 ml of phenol red, 0.5-3.5 g of agar or agarose and bacteriostat.EFFECT: reduced sensitivity test time, increased biologic safety during the said method implementation.14 cl, 4 dwg |
