发明名称 |
METHOD FOR MEASURING BONDING ACTIVITY OF ANTIBODY WITH AN ANTIGEN AND WITH AN Fc RECEPTOR |
摘要 |
<p>The present invention provides a simple method which is capable of evaluating the binding activities of an antibody to both an antigen and an Fc receptor. Disclosed is a method of measuring the binding activities of an antibody to both an antigen or antigen epitope and an Fc receptor or a fragment thereof, comprising a step of mixing the antibody with the antigen or antigen epitope labeled with one member of a set of donor and acceptor capable of fluorescent resonance energy transfer and the Fc receptor or fragment thereof labeled with the other member of the set of donor and acceptor; a step of irradiating the resultant mixture with light having a wavelength capable of exciting the donor; and a step of measuring the fluorescence level of the mixture. Also provided are a method of estimating the ADCC activity of an antibody, a method of controlling the quality of an antibody, a method of manufacturing an antibody, a method of screening for antibodies, and kits for use in these methods.</p> |
申请公布号 |
EP2169404(B1) |
申请公布日期 |
2016.01.27 |
申请号 |
EP20080777287 |
申请日期 |
2008.06.17 |
申请人 |
CHUGAI SEIYAKU KABUSHIKI KAISHA |
发明人 |
SAKITA, MASASHI;YOSHIMORI, TAKAYUKI |
分类号 |
G01N33/53;C07K16/00;G01N33/542 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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