| 主权项 |
1. A method of measuring a dynamic change in a profile of viable T cells, comprising:
performing a first contacting step at a first time point, wherein the first contacting step comprises contacting a slab with a first substrate, wherein the slab contains an array of microwells, each microwell dimensioned to hold a subnanoliter volume of liquid, wherein microwells in the array of microwells contain viable T cells and products secreted from said T cells, and, on average, contain no more than one single T cell or a few T cells per microwell; wherein the first substrate is pretreated with a plurality of first detection agents, wherein said plurality of first detection agents comprises first detection agents that respectively bind to different cytokines, whereby cytokines secreted by said T cells that are present in said microwells and are binding partners of a first detection agent are bound to the first detection agent in regions on the first substrate contacted by contents of the microwells, to yield a first printed array; after step (a), performing a second contacting step at a second time point, which second contacting step comprises contacting the slab with a second substrate, wherein the second substrate is pretreated with a plurality of second detection agents, wherein said plurality of second detection agents comprises second detection agents that are identical to said first detection agents that respectively bind to different cytokines, whereby cytokines secreted by said T cells that are present in said microwells and are binding partners of a second detection agent are bound to the second detection agent in regions on the second substrate contacted by contents of the microwells, to yield a second printed array; quantifying levels of said different cytokines bound to the first printed array and levels of said different cytokines bound to the second printed array, and determining rates of secretion of the different cytokines bound to the first printed array and rates of secretion of the different cytokines bound to the second printed array, thereby measuring a dynamic change in a profile of the T cells. |
