Lateral flow assay systems and methods

摘要

In one aspect, a diagnostic test system includes a housing, a reader, and a data analyzer. The housing includes a port for receiving a test strip. The reader obtains separable light intensity measurements from localized regions of an area of the detection zone exposed for optical inspection, wherein each of the localized regions is characterized by at least one surface dimension smaller than the first dimension. The data analyzer identifies ones of the light intensity measurements obtained from the at least one test region and computes at least one parameter from the identified ones of the light intensity measurements. In another aspect, the reader obtains a respective set of light intensity measurements from each of multiple corresponding regions of the exposed surface area of the detection zone, and the data analyzer computes at least one parameter from at least one of the sets of light intensity measurements.

主权项

1. A diagnostic test system, comprising:
a test strip comprising a detection zone containing an immobilized substance that binds a target analyte, wherein the detection zone comprises one or more test regions and one or more control regions; a reader that obtains separable light intensity measurements from multiple localized regions of the one or more test regions and the one or more control regions, wherein the reader comprises:
a light source that illuminates the multiple localized regions of the one or more test regions and the one or more control regions; anda light detector array that comprises individual light detectors, wherein the individual light detectors each obtain a separable light intensity measurement from one of the multiple localized regions of the one or more test regions and the one or more control regions; and a data analyzer comprising software that identifies one or more of the separable light intensity measurements obtained from the one or more test regions and the one or more control regions and computes at least one parameter from the identified one or more separable light intensity measurements.