Biosensor and method of making same

摘要

Sensors suitable for the sensing/detection of biological or chemical agents may be fabricated by immobilizing biological and/or chemical recognition components (selectors or probes) on a substrate by the polymerization of a suitable monomer in the presence of the selectors or probes, for example, by Polysiloxane Monolayer Immobilization (PMI). PMI may involve the polymerization of polysiloxane onto a substrate, onto which selector molecules are adsorbed or otherwise immobilized. The resulting immobilized selector molecule may then be used to interact with specific molecules (targets) within a mixture of molecules, thereby enabling those specific molecules to be detected and/or quantified.

主权项

1. A sensor fabrication method comprising the steps of:
a) polymerizing a layer of polysiloxane onto a substrate such that it sufficiently adheres thereto, wherein the substrate is selected from a group consisting of electrodes, diodes, field effect transistors, insulated gate field effect transistors, and metal oxide semiconductor field effect transistor; and b) adsorbing heptapeptide ligand selector molecules onto the layer of polysiloxane, wherein the steps of polymerizing and adsorbing occur concurrently and absent from an electrical voltage or current applied to the substrate and comprise: immersing the substrate in a solution or a suspension including polysiloxane-forming monomers and selector molecules, both polysiloxane-forming monomers and selector molecules being dispersed in the bulk of the solution or suspension, and wherein the polymerizing step and the adsorbing step hold heptapeptide ligand selector molecules onto the substrate by using hydrophobic silanol groups of the polysiloxane layer.