Fast focusing method and device for multi-spectral imaging

摘要

Fast focusing methods and devices for multi-spectral imaging are disclosed. The method comprising selecting one of a plurality of imaging channel as a reference channel, adjusting rotation positions of a stepper motor, calculating focus measures corresponding to all rotation positions of the stepper motor, and obtaining a first distribution curve; in each of the other imaging channels, selecting at least three rotation positions of the stepper motor, matching focus measures at the selected rotation positions with the first distribution curve to obtain a second distribution curve and a offset value between the first distribution curve and the second distribution curve, and calculating a clear focusing position of the imaging channel to be focused according to the offset value; performing a fine-tuning focusing, and thereby obtaining a more precise clear focusing position. A fast focusing for multi-spectral imaging and obtain clear multi-spectral images is obtained.

主权项

1. A fast focusing method for multi-spectral imaging comprising following steps:
S1, selecting one of a plurality of imaging channels as a reference channel, adjusting rotation positions of a stepper motor, calculating focus measures corresponding to all rotation positions of the stepper motor, and obtaining a first distribution curve that represents a change of the focus measure of the reference channel along with the rotation position of the stepper motor; S2, for each of the other imaging channels, selecting at least three rotation positions of the stepper motor, matching focus measures at the selected rotation positions with the first distribution curve to obtain a second distribution curve that represents a change of the focus measure of the imaging channel to be focused along with the rotation position of the stepper motor and an offset value between the first distribution curve and the second distribution curve, and calculating a clear focusing position of the imaging channel to be focused according to the offset value; S3, performing a fine-tuning focusing operation centering on the calculated clear focusing position by using a minimal rotation interval of the stepper motor, and thereby obtaining a precise clear focusing position.