HER2/neu-specific antibodies and methods of using the same

摘要

This invention relates to antibodies that specifically bind HER2/neu, and particularly chimeric 4D5 antibodies to HER2/neu, which have reduced glycosylation as compared to known 4D5 antibodies. The invention also relates to methods of using the 4D5 antibodies and compositions comprising them in the diagnosis, prognosis and therapy of diseases such as cancer, autoimmune diseases, inflammatory disorders, and infectious disease.

主权项

1. A method of treating a HER2/neu-expressing cancer in a patient having a cancer, comprising administering to said patient a therapeutically effective amount of an isolated polypeptide that binds human HER2/neu and comprises:
(I) a chimeric 4D5 immunoglobulin light chain variable domain comprising the amino acid sequence of SEQ ID NO: 4; and (II) an immunoglobulin heavy chain comprising an amino acid sequence selected from the group consisting of SEQ ID NO: 9, SEQ ID NO: 11, and SEQ ID NO: 13.