Yeast cell wall particles for receptor-targeted nanoparticle delivery

摘要

The present invention generally relates to yeast cell wall microparticles loaded with nanoparticles for receptor-targeted nanoparticle delivery. In particular, the present invention relates to trapping nanoparticles either on the surface or inside a yeast glucan particles, for example, yeast glucal particles. The present invention further relates to methods of making the yeast cell wall particles loaded with nanoparticles. The present invention also relates to methods of using the yeast cell wall particles loaded with nanoparticles for receptor-targeted delivery of the nanoparticles, e.g., drug containing nanoparticles.

主权项

1. A method for making a YCWP-NP delivery system, comprising yeast cell wall particles (YCWPs) comprising nanoparticles (NPs) having at least one dimension of about 1-40 nm contained within the interior of the YCWPs, the method comprising the steps of:
(a) loading the NPs into the YCWPs, wherein the loading comprises incubating a suspension comprising YCWPs and NPs for a time sufficient for the NPs to enter into the YCWPs, (b) drying the material resulting from (a), (c) optionally repeating steps (a)-(b) at least once, (d) resuspending the YCWPs and nanoparticles in a solvent for a time sufficient for additional NPs to enter into the YCWPs, (e) optionally drying the material resulting from (d), and (f) optionally trapping the NPs in the YCWPs using an appropriate trapping means, such that the YCWP-NP delivery system is made; wherein the NPs are insoluble in water.