SMS probe and SEM imaging system and methods of use

摘要

SMS probe imaging systems, methods of use thereof, and the like are disclosed. Embodiments of the present disclosure can use direct interrogation of objects (e.g., cells or tissue) within a small pool/droplet of liquid, optional thermal, mechanical, electrical, optical and chemical manipulation, followed immediately by liquid sampling, optional sample conditioning, and soft ionization of biomolecules.

主权项

1. A method, comprising:
imaging an object in a system to produce an object image; positioning an SMS probe and the object relative to one another at a first position using the object image; extracting a sample in the first position using the SMS probe, wherein the sample includes one or more components; and ionizing one or more of the components, wherein imaging is conducted using a scanning electron microscopy system; the method further comprising: forming a pool of a liquid on the object; wherein positioning includes positioning the SMS probe and the pool relative to one another, and wherein extracting includes extracting the sample from: the pool around the object, the surface of the object, within the object, or a combination thereof.