Cytotoxic anti-LAG-3 monoclonal antibody and its use in the treatment or prevention of organ transplant rejection and autoimmune disease

摘要

Cytotoxic anti-LAG-3 monoclonal antibodies or fragments thereof causing depletion of LAG-3+ activated T cells are described, as are related pharmaceuticals and methods of treating. Also described are related nucleic acid and protein sequences.

主权项

1. Monoclonal antibody IMP731 or a biologically active fragment thereof, wherein the monoclonal antibody IMP731 or the biologically active fragment thereof comprises a light chain kappa region as set forth in amino acids 21 to 240 of SEQ ID NO:13 and a heavy chain gamma region as set forth in amino acids 20 to 465 of SEQ ID NO:16, and wherein the monoclonal antibody IMP731 or biologically active fragment thereof comprises an Fc fragment from human IgGl, human IgM, or mouse IgG2a and an Fab fragment that binds LaG-3 protein, and wherein said moloclonal antibody IMP731 or biologically active fragment thereof is capable of depleting LAG-3+ activated T cells in a complement dependent cytotoxicity (CDC) assay, or in an antibody-dependent cell cytotoxicity (ADCC) assay, or both.