GENE SIGNATURES TO IDENTIFY MOLECULAR SUBGROUPS IN MEDULLOBLASTOMA TUMORS

摘要

Described herein are methods for determining the subgroup of medulloblastoma in a subject in need thereof. The subgroups of medulloblastoma include Group 3, Group 4, WNT and SHH. The subjects are children diagnosed with medulloblastoma or children suspected of having medulloblastoma.

主权项

1. A process, comprising providing a first composition comprising a plurality of isolated nucleic acids probes;
contacting the first composition to an RNA sample from a mammalian subject desiring a determination of a subgroup of medulloblastoma, to produce one or more cDNA molecules; providing a second composition comprising one or more isolated nucleic acids probes comprising a sequence capable of hybridizing to one or more nucleic acids selected from the group of genes consisting of βALCAM, βBCAT1, βCBLN3, βCD163, βCD4, βCSF1R, βCXCR4, βDKK2, βEGR1, βEOMES, βFOXG1, βFSTL5, βGABRA5, βGLI1, βHHIP, βIMPG2, βMMD, βMPP3, βMYC, βNPR3, βOTX2, βPDGFRA, βPDLIM3, βPID1, βPPP1R17, βPYGL, βSFRP1, βSLC6A5, βTERC, βTNC, βWIF1 or a combination thereof; contacting the second composition with the one or more cDNA molecules to amplify the one or more cDNA molecules; quantifying the expression level of the one or more genes to determine the subgroup of medulloblastoma to which the sample belongs; and computing the prediction probabilities of the sample belonging to the subgroup of medulloblastoma.