SUBTYPING LUNG CANCERS

摘要

This disclosure concerns the identification of biomarkers that are characteristic of squamous or non squamous (e.g., adenocarcinoma, large cell carcinoma, carcinoid tumor, sarcomatoid carcinoma) subtypes of non small cell lung cancer (NSCLC), clinically useful NSCLC classifiers, kits and arrays for distinguishing squamous and nonsquamous NSCLC subtypes, bioinformatic methods for determining clinically useful classifiers, and methods of use of each of the foregoing.

主权项

1. A method of characterizing a lung sample obtained from a subject, comprising:
obtaining from the sample raw expression values for each of at least two biomarkers in Table 2, 3, or 4 and at least one normalization biomarker(s); normalizing the raw expression values for each of the at least two biomarkers in Table 2, 3, or 4 to the raw expression values for the at least one normalization biomarker(s) to produce normalized expression values for each of the at least two biomarkers in Table 2, 3, or 4; combining the normalized expression values for each of the at least two biomarkers in Table 2, 3, or 4 to generate an output value; comparing the output value to a cut-off value, wherein the cut-off value was determined by regression analysis of normalized expression values for the at least two biomarkers in Table 2, 3, or 4 in a plurality of NSCLC samples known in advance to be squamous cell NSCLC or nonsquamous cell NSCLC; and characterizing the sample as squamous cell NSCLC if the output value is on the same side of the cut-off value as the plurality of known squamous cell NSCLC samples or characterizing the sample as nonsquamous cell NSCLC if the output value is on the same side of the cut-off value as the plurality of known nonsquamous cell NSCLC samples.