A METHOD OF MEASURING ANTIBODY CONCENTRATION IN A SAMPLE

摘要

A method for determining antibody concentration in a liquid sample comprising the steps of incubating in a reaction chamber the liquid sample with an antibody-binding probe comprising a generic antibody binding protein conjugated to a long life fluorescent dye to provide a reaction mixture, assaying the reaction mixture in the reaction chamber for fluorescence polarisation to detect a change in polarisation between excitation and emission light, and correlating the change in polarisation with antibody concentration of the sample.