| 摘要 |
PROBLEM TO BE SOLVED: To provide a method and kit for highly precise DNA typing, in which ambiguity derived from phase ambiguity is eliminated.SOLUTION: The present invention provides a method for the DNA typing of HLA, which is characterized by comprising: (1) preparing a primer set which can respectively anneal specifically to an upstream region and a downstream region of each of HLA-A, HLA-B, HLA-C, HLA-DRB1, HLA-DPB1 and HLA-DQB1 genes in human genome nucleotide sequences, (2) carrying out the PCR amplification of a sample to be tested (DNA) using the primer set; (3) determining a base sequence for a PCR-amplified product; and (4) carrying out a homology search against a database. |
