TUNABLE CONTROL OF PROTEIN DEGRADATION IN SYNTHETIC AND ENDOGENOUS BACTERIAL SYSTEMS

摘要

The methods and compositions described herein relate, in part, to the generation of a synthetic degradation system in E. coli that provides tunable control of the protein level of targeted genes by using components of the Mesoplasma florum tmRNA system. Provided herein are degradation tag variants that permit independent control of both the initial level and inducible degradation rate of attached proteins.

主权项

1. A composition comprising a modified protein degradation tag derived from a Mesoplasma florum degradation tag, wherein the modified degradation tag comprises altered degradation dynamics compared to the unmodified Mesoplasma florum degradation tag.