| 主权项 |
1. A method of culturing a mammalian cell, the method comprising:
providing a shake tube containing a mammalian cell disposed in a first liquid culture medium, wherein the first liquid culture medium occupies about 10% to about 30% of the volume of the shake tube and contains a plurality of microcarriers at a concentration of about 1.0 g/L to about 15.0 g/L; incubating the shake tube for a period of time at about 32° C. to about 39° C. and with a rotary agitation of about 120 revolutions per minute (RPM) to about 240 RPM; and after about the first 48 to 96 hours of the period of time, continuously or periodically removing a first volume of the first liquid culture medium and adding to the first liquid culture medium a second volume of a second liquid culture medium, wherein the first and second volumes are about equal. |
