PHOTOCLEAVABLE DEOXYNUCLEOTIDES WITH HIGH-RESOLUTION CONTROL OF DEPROTECTION KINETICS

摘要

Provided herein are new classes of photocleavable deoxynucleotides that allow for more precise control over deprotection kinetics compared to previously described compounds. The compounds further feature more favorable solubility properties. The nucleotides find use in methods such as next-generation sequencing. A series of molecules are provided with defined organic substituents that allow fine tuning of the deprotection kinetics when irradiated with an appropriate light source.