摘要 |
The invention relates to a polypeptide comprising an amino acid having a cyclopropene group wherein said cyclopropene group is joined to the amino acid via a carbamate group. Suitably the cyclopropene group is a 1,3-disubstituted cyclopropene such as a 1,3-dimethylcyclopropene. Suitably the cyclopropene group is present as a residue of a lysine amino acid. Preferably, the amino acid comprises Nε-[((2-methylcycloprop-2-en-1-yl)methoxy)carbonyl]-1-lysine. The invention also relates to a method of making the polypeptide by genetically incorporating an amino acid comprising a cyclopropene group joined to the amino acid via a carbamate group, into a polypeptide. The invention also relates to an amino acid comprising cyclopropene wherein said cyclopropene group is joined to the amino acid moiety via a carbamate group. The invention yet further relates to an antibody drug conjugate (ADC) comprising said polypeptide. |