发明名称 |
METHOD AND APPARATUS FOR DISCRIMINATION OF UNDIFFERENTIATED PLYRIPOTENT STEM CELL, AND AUTOMATIC CULTURE METHOD AND APPARATUS |
摘要 |
Provided are a method capable of discriminating between a colony of differentiated pluripotent stem cells and a colony of undifferentiated pluripotent stem cells, and a method for automatically culturing undifferentiated pluripotent stem cells and a system thereof. A differentiated colony composed of differentiated pluripotent stem cells, an undifferentiated colony composed of undifferentiated pluripotent stem cells and a multilayered colony having pluripotent stem cells stacked in multiple layers are discriminated by processing a photo image of a colony including pluripotent stem cells in a culture vessel. Specifically, a colony having a luminance higher than a first threshold A is determined as a differentiated colony, a colony having a luminance equal to or lower than the first threshold A and equal to or higher than a second threshold B is determined as an undifferentiated colony, and a colony having a luminance lower than the second threshold B is determined as a multilayered colony. |
申请公布号 |
EP2487249(A4) |
申请公布日期 |
2016.01.20 |
申请号 |
EP20100821752 |
申请日期 |
2010.10.07 |
申请人 |
KAWASAKI JUKOGYO KABUSHIKI KAISHA |
发明人 |
WATAKABE, KEIZO;NAKAMURA, YOHICHI;OHJI, OSAMU;NAKASHIMA, KATSUMI |
分类号 |
C12Q1/04;C12M1/34;C12M3/00;C12N5/07;G06K9/00 |
主分类号 |
C12Q1/04 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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