| 摘要 |
<p>Proteinaceous hormones of animal origin are activated for administration to humans by treatment with proteolytic enzymes to a desired degree of hydrolysis, followed by inactivation of the enzyme by means of a non-toxic inhibiting substance specific to the enzyme used. The animal hormone may be pituitary gland hormones, especially the somatotropic hormone. The inhibitors may be (a) vegetable e.g. lima bean or soy bean inhibitor; (b) animal e.g. ovomucoids, peptidase inhibitors extracted from pancreas porotids or ascaris; or (c) chemical e.g. diisofluorophosphates. The required degree of hydrolysis is 25 to 35% of the total hydrolysis. The inhibitor terminates the hydrolysis and inhibits further enzyme activity of the hormone, even when the product is administered with separation of the inhibitor/enzyme complex. The complex may be removed by dialysis at pH 6-8 and removal of the precipitated active components.</p> |
