发明名称 |
PROTOCOL FOR IDENTIFYING AND ISOLATING ANTIGEN-SPECIFIC B CELLS AND PRODUCING ANTIBODIES TO DESIRED ANTIGENS |
摘要 |
Methods of identifying antigen-specific antibody-secreting and antibody-forming cells, such as antigen-specific B cells, and methods for cloning the antigen-specific antibody sequences of the antibody produced by these cells are provided. In particular, the methods include enriching B cells for antigen-specific B cells, culturing the antigen-specific B cells to generate clonal B cell populations, detecting clonal B cells that produce a single antigen-specific antibody, optionally screening the clonal B cell populations for functional activity, staining and sorting the cells to isolate the antigen-specific B cells, sequencing the nucleic acids encoding the antigen-specific antibody sequences, expressing the sequences to produce an antibody, isolating the antibody and screening the antibody for antigen recognition. The methods provide improved enrichment and selection of antigen-specific antibody-secreting and antibody-forming cells, which enhances recovery of antigen-specific antibodies. |
申请公布号 |
EP2972330(A2) |
申请公布日期 |
2016.01.20 |
申请号 |
EP20140764593 |
申请日期 |
2014.03.18 |
申请人 |
ALDER BIOPHARMACEUTICALS, INC. |
发明人 |
ALLISON, DANIEL, S.;DUTZAR, BENJAMIN, H.;GARCIA-MARTINEZ, LEON, F.;ANDERSON, KATIE;OJALA, ETHAN, W.;LATHAM, JOHN, A.;BILLGREN, JENS;JENSEN, ANNE, ELISABETH CARVALHO |
分类号 |
G01N33/50;C07K16/18;C07K16/22;C07K16/40;G01N33/569 |
主分类号 |
G01N33/50 |
代理机构 |
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代理人 |
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地址 |
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