| 摘要 |
The invention relates to a method of separating a biomolecule of interest from one or more contaminant species comprising contacting a buffer-equilibrated apatite matrix with a sample containing the biomolecule of interest and the one or more contaminant species, washing the matrix with an apatite matrix wash solution and collecting fractions of the apatite matrix flow-through which contain the biomolecule of interest and wherein the biomolecule of interest is separated from the one or more contaminant species; wherein upon contact with and transit through the apatite matrix the biomolecule and the one or more contaminant species do not become adsorbed to the matrix and wherein no desorption is performed; and wherein the biomolecule of interest is separated from the one or more contaminant species following differential rate of transit of the biomolecule and the one or more contaminant species through the apatite matrix. Also claimed is a method for producing a purified biomolecule solution from a contaminated biomolecule solution comprising using: i) ion exchange matrix chromatography, and ii) an apatite matrix separation step. Also claimed are associated kits containing an apatite matrix and an ion exchange matrix. Preferably, the apatite matrix is hydroxyapatite. |
