Methods and compositions for regulating RNA import into mitochondria

摘要

Disclosed herein are methods of manipulating the processing, targeting, and/or internalization of a nucleic acid molecule. Also disclosed are recombinant and/or isolated nucleic acid molecules having a first nucleic acid sequence, which is a wild-type sequence or an altered sequence, directly or indirectly linked to a second nucleic acid sequence which is a mitochondria localization sequence, an RNA import sequence, or a combination thereof, and methods of using thereof.

主权项

1. A method of manipulating the processing, targeting, and/or internalization of a nucleic acid molecule which comprises one or more of the following steps
providing an altered tRNA aminoacyl stem that is directly or indirectly linked to the nucleic acid molecule to thereby enable the nucleic acid molecule to escape the nucleus of a cell; providing a mitochondria localization sequence that is directly or indirectly linked to the nucleic acid molecule to thereby cause the nucleic acid molecule to localize in the proximity of a mitochondrion; and providing an RNA import sequence that is directly or indirectly linked to the nucleic acid molecule to thereby cause the nucleic acid molecule to be internalized by a mitochondrion, and wherein the RNA import sequence is selected from the group consisting of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:38, SEQ ID NO:39, sequences which are about 15-30 nucleotides long and comprise SEQ ID NO:39, and sequences complementary thereto.