Spectroscopic sample inspection for automated chromatography

摘要

A method and a system for generating an extraction sample from a dried sample spot. The method includes acquiring a spectroscopic image of an analyte in the spot. The spectroscopic image includes information on a spatial distribution of the analyte. A region of the spot to be extracted is determined from the spectroscopic image and an extraction sample is generated from the determined region. In other embodiments, the method and system are based on acquiring a spectroscopic measurement of an analyte in the dried sample spot, determining a quantity of the analyte in the spot based on the spectroscopic measurement and determining a volume of an extraction solvent for creating an extraction sample from the spot. The determined volume is based on the determined quantity of the analyte and a detection characteristic of an analytical measurement system. In other embodiments, the method is applied to solid samples.

主权项

1. A method of generating an extraction sample from a dried sample spot, the method comprising:
acquiring a spectroscopic image of an analyte in a dried sample spot, the spectroscopic image comprising spectroscopic data that are dependent on location within the dried sample spot based on a concentration of the analyte according to location within the dried sample spot; determining a region of the dried sample spot for extraction based upon the spectroscopic image; and generating an extraction sample from the determined region of the dried sample spot.