Method for producing terpenes

摘要

The present invention relates to a method for producing terpenes in fungi, wherein a terpene biosynthetic gene cluster having terpene biosynthetic genes and regulatory regions operably linked to said genes is activated. The invention relates also to a terpene biosynthetic gene duster and regulatory regions of such terpene biosynthetic gene cluster usable is production of terpenes, use of regulator for regulating the terpene production and use of Aspergillus nidulans FGSC A4 for producing terpenes. The method of invention provides higher yields of enriched terpene product without essential amount of side-products.

主权项

1. A method for producing terpenes in fungi, comprising the steps of:
(a) providing a nucleic acid encoding a transcription factor, wherein said transcription factor activates a terpene biosynthetic gene cluster, and wherein said transcription factor comprises a polypeptide having at least 90% sequence identity to SEQ ID NO: 74; (b) operably linking said nucleic acid to a promoter, to produce an expression construct; (c) transforming said expression construct into a host cell carrying a terpene biosynthetic gene cluster; and (d) cultivating said host cell under conditions which allow expression of the transcription factor, and which allow activation of the terpene biosynthetic gene cluster by the transcription factor.