siRNA phospholipid conjugate

摘要

siRNA-conjugated liposomes and micelles, methods of making such conjugates, and methods of using such conjugates, such as for the delivery of siRNA to cells to reduce expression of target polypeptides in such cells, are described.

主权项

1. A composition comprising:
a micelle or a liposome, the micelle or the liposome comprising: phospholipids; an siRNA reversibly linked to a first phospholipid, wherein the siRNA-phospholipid conjugate is part of the layer structure of the micelle or the liposome, wherein the siRNA is linked to the phospholipid by a reversible disulfide bond, and polyethylene glycol (PEG) conjugated to an additional phospholipid of the micelle or the liposome, wherein weight ratio of the siRNA-phospholipid conjugate to the PEG-phospholipid conjugate is about 1:200 to about 1:750.