Cellulase enzyme mixtures for depilling and uses thereof

摘要

The present invention relates to a depilling composition comprising an enzyme mixture that comprises a Family 45 cellulase and one or more additional cellulases selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof. The enzyme mixture may be characterized in that the Family 45 and Family 5 cellulases or the Family 45 and Family 6 cellulases are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity. The enzyme mixture may be secreted by a genetically modified microbe overexpressing the foregoing cellulases. Also provided is a process for depilling that comprises a step of contacting cellulose-containing goods with the depilling composition.

主权项

1. A depilling composition comprising an enzyme mixture, which enzyme mixture comprises a Family 45 cellulase and one or more additional cellulases selected from a Family 5 cellulase, a Family 6 cellulase or a combination thereof, wherein the enzyme mixture is secreted by a genetically modified microbe overexpressing (a) a gene encoding the Family 45 cellulase; and (b) a gene or genes encoding the one or more additional cellulases, wherein the Family 45 cellulase has at least 90% sequence identity to the sequence of amino acids 1-213 of SEQ ID NO:7 (HiCel45) or to the sequence of amino acids 1-166 of SEQ ID NO:4 (TrCel45), wherein the enzyme mixture is characterized in that the Family 45 and the Family 5 cellulases or the Family 45 and the Family 6 cellulases are present at a weight ratio that exhibits synergy in an assay that measures specific depilling activity.