| 发明名称 |
METHOD OF INDUCING DIFFERENTIATION FROM PLURIPOTENT STEM CELLS TO GERM CELLS |
| 摘要 |
This invention provides a method of producing a primordial germ cell-like cell (PGCLC) from an epiblast isolated from an embryo or an epiblast-like cell (EpiLC) induced from a pluripotent stem cell (PSC), which comprises allowing the epiblast or EpiLC to express exogenous transcription factor(s) selected from the group consisting of: (i) Blimp1, Prdm14 and Tfap2c; (ii) Blimp1 and Prdm14; (iii) Blimp1 and Tfap2c; (iv) Prdm14 and Tfap2c; and (v) Prdm14; thereby inducing the epiblast or EpiLC into a PGC state without acquiring transient mesodermal program. |
| 申请公布号 |
US2016010056(A1) |
申请公布日期 |
2016.01.14 |
| 申请号 |
US201414771287 |
申请日期 |
2014.02.28 |
| 申请人 |
KYOTO UNIVERSITY |
发明人 |
NAKAKI Fumio;SAITOU Mitinori |
| 分类号 |
C12N5/0735 |
主分类号 |
C12N5/0735 |
| 代理机构 |
|
代理人 |
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| 主权项 |
1. A method of producing a primordial germ cell-like cell (PGCLC) from an isolated epiblast or epiblast-like cell (EpiLC), which comprises allowing the epiblast or EpiLC to express exogenous transcription factor(s) selected from the group consisting of:
(i) Blimp1, Prdm14 and Tfap2c; (ii) Blimp1 and Prdm14; (iii) Blimp1 and Tfap2c; (iv) Prdm14 and Tfap2c; and (v) Prdm14;thereby inducing the epiblast or EpiLC into a PGC state without acquiring transient mesodermal program. |
| 地址 |
Kyoto-shi, Kyoto JP |
