Tetrary Gene Delivery System for Gene Therpay and Methods of Its Use

摘要

Delivery systems and their methods of formation are generally provided. The delivery system can protect a delivery molecule (e.g., DNA/RNA) and deliver it into a cell via serum. In one embodiment, the method can include binding a delivery molecule with polyethylenimine such that an end of the delivery molecule is exposed; capping the end of the delivery molecule with a first biocompatible polymer to form a core; and encapsulating the core with a second biocompatible polymer. The resulting delivery system can include a delivery molecule bonded with polyethylenimine such that an end of the delivery molecule is exposed; a first biocompatible polymer electrostatically bonded to the end of the delivery molecule to form a core; and a second biocompatible polymer encapsulating the core.

主权项

1. A delivery system, comprising:
a delivery molecule bonded with polyethylenimine such that an end of the delivery molecule is external to the polyethylenimine, wherein the polyethylenimine has a positive electrostatic charge, and wherein the end of the delivery molecule has a negative electrostatic charge; a crosslinked polyspermine capping the end of the delivery molecule, the crosslinked polyspermine having an N/P ratio greater than 5 and wherein the crosslinked polyspermine comprises disulfide bonds as crosslinks, the crosslinked polyspermine having a positive electrostatic charge such that the crosslinked polyspermine is electrostatically bonded to the end of the delivery molecule and the system comprises a core that includes the polyethylenimine, the delivery molecule, and the crosslinked polyspermine; and hyaluronic acid or alginate encapsulating the core, wherein the hyaluronic acid or alginate has a negative electrostatic charge, and wherein the hyaluronic acid or alginate is electrostatically bonded to the core, wherein the hyaluronic acid or alginate is present in a weight ratio of up to 50 compared to the weight of the delivery molecule.