FUSION PROTEIN COMPRISING TARGETING MOIETY, CLEAVAGE SITE, AND CELL MEMBRANE PENETRATING DOMAIN, AND USE THEREOF

摘要

A fusion protein including a targeting moiety, a cleavage site, and a cell membrane penetrating domain, a conjugate including the fusion protein and a bioactive molecule, and methods employing the fusion protein and the conjugate are provided.

主权项

1. A fusion protein comprising a targeting moiety, a cleavage site, and a cell membrane penetrating domain, wherein
the targeting moiety is an antibody, antigen-binding antibody fragment, or protein scaffold that specifically binds to a cell-surface receptor; the cleavage site is a protease or peptidase recognition site; and the cell membrane penetrating domain is a membrane-translocation sequence, a macromolecule intracellular transduction domain, a fusion peptide comprising a hydrophobic peptide and a basic peptide, or a combination thereof.